Friday, June 19, 2009

Pseudomonas fulva????

The National Centre for Cellular Sciences (NCCS), Pune finally released the genotypic identification results of my bacteria 2 days ago and to say it was surprising would be an understatement! While all of us were expecting it to belong to the genus Lampropedia (Which was my find from the huge Bacteriology manual btw), the result gave it a 99% match to Pseudomonas fulva- A pretty new species given that no one around had any clue about it. For those who are interested, here is the genetic composition of the bacterium:-

GCGGTCACTTAATCGCGTTAGCTGCGCCACTAAAATCTCAAGGATTCCAACGGCTAG

TTGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGC

TTTCGCACCTCAGTGTCAGTATCAGTCCAGGTGGTCGCCTTCGCCACTGGTGTTCCT

TCCTATATCTACGCATTTCACCGCTACACAGGAAATTCCACCACCCTCTACCGTACTC

TAGCTTGCCAGTTTTGGATGCAGTTCCCAGGTTGAGCCCGGGGCTTTCACATCCAA

CTTAACAAACCACCTACGCGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC

CTCTGTATTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAA

CGTCAAAATTGCAAAGTATTAATCTACAGCCCTTCCTCCCAACTTAAAGTGCTTTAC

AATCCGAAGACCTTCTTCACACACGCGGCATGGCTGGATCAGGCTTTCGCCCATTG

TCCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGT

GTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTTGGTGAGCCATTACC

TCACCAACTAGCTAATCCGACCTAGGCTCATCTGATAGCGCAAGGCCCGAAGGTCC

CCTGCTTTCTCCCGTAGGACGTATGCGGTATTAGCGTTCCTTTCGAAACGTTGTCC

CCCACTACCAGGCAGATTCCTAGGCATTACTCACCCGTCCGCCGCTGAATCAAGGAG

CAAGCTCCCGTCATCCGCTCGACTTGCATGTGTTAGCCTGCCGCCAGCGTCAATCTG

Even mightygoogle has no useful info on this but I suppose its clear now that I got pics to compare:-

Pseudomonas literature Picture


My bacterium observed under Microscope


While that may sound gibberish, it was big enough to warrant an application to present this work at the National Conference on Biotechnology to be held on 1st and 2nd of July this year in Hyderabad. More on that if selected to appear!

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